Fig. 6

Induction of HDAC1 expression or p38MAPK signaling reversed the protective action of obacunone (OB) on chondrocyte damage. A Primary chondrocytes were transfected with HDAC1 or p38MAPK overexpression plasmids. The transfection efficiency was verified by performing western blotting. B Cell survival following transfection and the treatment of IL-1β and OB was detected by CCK-8 assay. C Western blotting was performed for detecting the protein expression of collagen II, ADAMTS-5, and MMP13 (D) Western blotting was performed for detecting the protein expression of IL-6, TNF-α, and IL-18. n = 3. Data were analyzed by one-way ANOVA followed by Tukey's post hoc test. **P<0.01 and ***P<0.001